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. 2017 Jan 17;7:40777. doi: 10.1038/srep40777

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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To validate the RNA-seq data, the relative mRNA levels of 15 randomly selected DEGs in the intestine of A. hydrophila-infected grass carp were examined by qPCR. The mRNA levels by qPCR are presented as the fold change compared with the mock-treated control after normalization against β-actin. The relative expression levels from the RNA-seq analysis were calculated as RPKM values. The asterisk (*) denotes the presence of a statistically significant difference (p < 0.05) in mRNA levels between the RNA-seq and qPCR analysis.