Table 1. Proteins with significantly decreased abundance at the critical node in 0-d, 3-d, and 4-d aged rice seeds.
| Spot | Protein name | Accession No. | Scores | Fold |
Carbonylation | |
|---|---|---|---|---|---|---|
| 0d/3d | 0d/4d | |||||
| Energy | ||||||
| D1 | Vacuolar proton-ATPase | NP_001058280.1 | 890 | 1.85 | 4.54 | C1 |
| D4 | Phospoglycerate mutase | NP_001044625.1 | 1094 | 1.59 | 2.74 | C4 |
| D5 | ATP synthase lipid-binding protein | YP_002000594.1 | 773 | 1.82 | 2.55 | C5 |
| D6 | ATP synthase lipid-binding protein | YP_002000594.1 | 744 | 1.71 | 3.01 | |
| D7 | 6-phosphogluconate dehydrogenase 1 | NC_029261.1 | 1067 | 2.93 | 5.99 | C6 |
| D8 | Pyruvate decarboxylase | BAC20138.1 | 446 | 1.49 | 2.29 | C7 |
| D14 | ATP synthase subunit beta | NP_001043900.1 | 1482 | 1.59 | 2.11 | C12 |
| D19 | Malate dehydrogenase | NP_001064860.1 | 903 | 1.35 | 30.02 | C16 |
| D22 | Cyt-RPEase | NP_001063604.2 | 187 | 1.93 | 5.13 | |
| D29 | Triosephosphate isomerase | AAB63603.1 | 689 | 1.29 | 3.06 | |
| D30 | Carboxymethylenebutenolidase-like protein | NP_001043244.1 | 536 | 1.16 | 1.42 | |
| D36 | Pyruvate decarboxylase 2 | NP_001049811.1 | 615 | 1.18 | 2,17 | C21 |
| D48 | Succinate dehydrogenase flavoprotein subunit | NP_001058845.1 | 123 | 1.26 | 1.82 | C27 |
| D53 | Triosephosphate isomerase | AAB63603.1 | 995 | 1.26 | 2.44 | C30 |
| D58 | Enolase | AAC49173.1 | 1075 | 1.79 | 1.98 | C31 |
| D61 | Pyruvate decarboxylase 1 | NC_029260.1 | 147 | 1.19 | 2.39 | |
| D62 | Phosphofructokinase beta subunit | NP_001057284.1 | 596 | 1.04 | 2.66 | |
| D63 | Beta-enolase | AAC49173.1 | 269 | 2.34 | 1.48 | |
| D64 | ADH1 | ADH03842.1 | 997 | 2.36 | 1.87 | C32 |
| D69 | Vacuolar ATP synthase 16 kDa proteolipid subunit | AAO72561.1 | 145 | ∞ | ∞ | |
| D70 | Ketol-acid reductoisomerase | NP_001043738.1 | 186 | ∞ | ∞ | |
| D74 | UDP-glucose 6-dehydrogenase 3 | NP_001051328.1 | 568 | ∞ | ∞ | |
| D78 | Glyceraldehyde-3-phosphate dehydrogenase 2, | NP_001053139.1 | 451 | ∞ | ∞ | |
| disease/defense | ||||||
| D2 | 70 kDa heat shock protein | ABF95267.1 | 502 | 1.51 | 2.33 | C2 |
| D13 | Chaperonin CPN60-1, mitochondrial | NP_001048938.1 | 248 | 2.40 | 3.24 | C11 |
| D26 | Dehydration stress-induced protein | NP_001064434.1 | 192 | 1.49 | 2.58 | |
| D38 | Salt tolerance protein 5 | NP_001057221.1 | 153 | 1.16 | 4.14 | C23 |
| D44 | 17.9 kDa class I heat shock protein | NP_001049657.1 | 686 | 1.06 | 1.51 | C25 |
| D46 | Glutathione S-transferase 2 | NP_001044339.1 | 282 | 1.39 | 2.46 | C26 |
| D52 | 18.0 kDa class II heat shock protein | NP_001042231.1 | 339 | 1.17 | 1.88 | |
| D54 | 70 kDa heat shock protein | ABA95501.2 | 813 | 1.49 | 2.55 | |
| D55 | 70 kDa heat shock protein | NP_001044757.1 | 725 | 1.46 | 2.42 | |
| D56 | Silver leaf whitefly-induced protein 1 | NP_001047794.1 | 653 | 1.38 | 1.50 | |
| D57 | L-ascorbate peroxidase 1 | NP_001049769.1 | 639 | 3.32 | 10.89 | |
| D59 | Cold shock domain protein 2 | NP_001060914.1 | 525 | 1.91 | 1.94 | |
| D60 | TCP-1/cpn60 chaperonin | AAT77033.1 | 338 | 1.28 | 1.82 | |
| D72 | Germin-like protein 8-2 | AAC04834.1 | 136 | ∞ | ∞ | |
| D73 | GDP-mannose 3,5-epimerase 2 | NP_001068183.1 | 290 | ∞ | ∞ | |
| D75 | Germin-like protein 8-2 | AAC04834.1 | 106 | ∞ | ∞ | |
| Metabolism | ||||||
| D10 | 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase | ABG22095.1 | 1087 | 2.89 | 4.92 | C9 |
| D15 | Glutamine synthetase cytosolic isozyme 1-1 | NP_001048045.1 | 328 | 1.88 | 3.78 | C13 |
| D16 | Reversibly glycosylated polypeptide | CAA77235.1 | 178 | 1.63 | 9.04 | |
| D17 | Methylmalonate semi-aldehyde dehydrogenase | NP_001059082.1 | 412 | 2.23 | 4.65 | C14 |
| D18 | Ketol-acid reductoisomerase | NP_001043738.1 | 663 | 1.50 | 4.47 | C15 |
| D20 | Reversibly glycosylated polypeptide | CAA77235.1 | 906 | 1.53 | 2.93 | C17 |
| D37 | Phosphoglucomutase | NP_001051066.1 | 470 | 1.09 | 6.36 | C22 |
| D41 | Proteasome subunit beta type-1 | NP_001063603.1 | 663 | 1.18 | 2.33 | |
| D47 | Inosine-5’-monophosphate dehydrogenase 1 | AAK09225.1 | 598 | 1.17 | 1.94 | |
| D49 | Leucyl-cystinyl aminopeptidase | Q6K669.1 | 1325 | 1.37 | 3.83 | C28 |
| D50 | S-adenosylmethionine synthase | P93438.1 | 638 | 1.61 | 2.21 | |
| protein synthesis | ||||||
| D32 | 60 S acidic ribosomal protein P0 | NP_001060923.1 | 531 | 1.09 | 2.49 | C19 |
| D40 | Guanine nucleotide-binding protein subunit beta | NP_001043910.1 | 1053 | 1.25 | 1.40 | |
| D65 | Bowman Birk trypsin inhibitor | BAD52869.1 | 107 | 1.37 | 1.52 | |
| D67 | Succinyl-CoA ligase [ADP-forming] subunit beta | NP_001047463.1 | 145 | ∞ | ∞ | |
| D68 | Mitochondrial processing peptidase beta subunit | NP_001049357.1 | 194 | ∞ | ∞ | |
| D76 | Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein | NP_001049884.1 | 106 | ∞ | ∞ | |
| protein destination and storage | ||||||
| D28 | Cupin family protein | AAS07324.1 | 394 | 1.66 | 2.97 | |
| D42 | Cupin family protein | ABF95817.1 | 202 | 1.21 | 2.92 | |
| D43 | Cupin family protein | ABF95817.1 | 376 | 1.31 | 1.68 | |
| D71 | Cupin family protein | ABF95817.1 | 236 | ∞ | ∞ | |
| D77 | Cupin family protein | ABF95817.1 | 144 | ∞ | ∞ | |
| Transcription | ||||||
| D21 | Elicitor-inducible protein EIG-J7 | NP_001048145.1 | 232 | 1.52 | 5.34 | |
| D35 | Glycine-rich RNA-binding protein 7 | AAT85299.1 | 464 | 1.29 | 3.72 | |
| D39 | Asparagine-tRNA ligase | NP_001043066.1 | 460 | 1.14 | 2.74 | C24 |
| D45 | Glycine-rich RNA-binding protein GRP1A | NP_001067344.1 | 64 | 1.49 | 1.66 | |
| growth/division | ||||||
| D12 | Actin | BAB63635.1 | 700 | 1.43 | 3.82 | C10 |
| D51 | Late embryogenesis abundant protein 1 | A2XG55.2 | 355 | 1.21 | 1.35 | C29 |
| D66 | Spermidine synthase 1 | NP_001059438.1 | 183 | ∞ | ∞ | |
| secondary metabolism | ||||||
| D31 | NADH-dependent enoyl-ACP reductase | NP_001061557.1 | 570 | 1.31 | 6.18 | |
| D34 | Lactoylglutathione lyase | NP_001055113.1 | 186 | 1.14 | 4.01 | |
| Transporters | ||||||
| D9 | ECF transporter A component EcfA | BAD11555.1 | 777 | 1.72 | 3.95 | C8 |
| signal transduction | ||||||
| D33 | GDP dissociation inhibitor | NP_001055566.1 | 550 | 1.71 | 2.61 | C20 |
| unclear classification | ||||||
| D3 | Os09g0491772 protein | NP_001175918.1 | 241 | 1.54 | 2.91 | C3 |
| D11 | OSJNBa0010H02.6 protein | NP_001053500.1 | 748 | 1.67 | 3.05 | |
| D23 | Uncharacterized protein | NP_001056364.1 | 803 | 1.32 | 1.90 | |
| D24 | OSJNBa0004N05.4 protein | CAE03380.1 | 608 | 1.42 | 2.44 | C18 |
| D25 | Uncharacterized protein P0435H01.4 | NP_001044131.1 | 654 | 1.53 | 2.07 | |
| D27 | Uncharacterized protein P0036D10.5 | NP_001174164.1 | 238 | 1.46 | 2.57 | |
Mascot scores >65 are statistically significant at p < 0.05.