Figure 5. Correlation between urine and plasma fumarase activity and fumarase activity with varying degrees of ischemia/reperfusion injury.

A deviation from zero was found between (A) Urine fumarase activity and fumarate/malate ratio (n = 6, p = 0.021, R² = 0.77.), and (B) Plasma fumarase activity and fumarate/malate ratio (n = 6, p = 0, R² = 0.722). The dashed line indicates the 95% confidence interval. The straight line indicates the regression line. Fumarase activity in 30-min/30-min IRI plasma (p = NS) and urine (p ≤ 0.001), in 20-min/1-day IRI plasma (p = NS) and urine (p = NS), in 40-min/1-day IRI plasma (p = NS) and urine (p = 0.0005), in 20-min/1-week IRI plasma (p = NS) and urine (p = 0.0001), in 40-min/1-week IRI plasma (p = NS) and urine (p ≤ 0.0001), in 40-min/1-day bilateral IRI plasma (p ≤ 0.0001) and urine (p = 0.0006), in 30-min/1-day IRI plasma (p = NS) and urine (p = 0.0003) and in 60-min/1-day IRI plasma (p = 0.0091) and urine (p = NS). In all examples lists, the length of the period of ischemia is given first, followed by that of the period of reperfusion. One-way ANOVA with a Holm-Sidak’s multiple comparisons test was used to compare values between the varying degrees of IRI. All relevant abbreviations as in Fig. 1. ANOVA = analysis of variance; NS = not statistically significant.