Table 1. Morphological Observations of the Isolated Acanthamoeba spp. Amongst Positive Samples on Non-Nutrient Agar Culture Media^a.

No.	Region	Cyst Diameter, μm	Groupb	Culture	PCR	Genotype
1	Gharakandc,d	11.1 ± 1.02	II	+	+	T4
2	Amirabadd	14.2 ± 0.82	II	+	+	-
3	Hamamiand	11.1 ± 1.2	III	+	+	-
4	Hossinmamed	12.1 ± 0.99	II	+	+	-
5	Miandoabe	11.8 ± 1.01	III	+	+	-
6	Kostehd	11.3 ± 1.01	III	+	+	T4
7	Gharajozd	11.1 ± 0.95	II	+	+	-
8	Miandoab2d	15.2 ± 1.08	III	+	+	T5
9	Khankandie	13.3 ± 1.04	II	+	+	T4
10	Mlalare	12.2 ± 1.02	II	+	+	T4
11	Tazeghallad	14.2 ± 0.97	II	+	+	T4
12	Akhtatard	12.3 ± 1.1	II	+	+	T4
13	Shahrikandd	11.5 ± 0.89	II	+	+	T4
14	Siminehd	12.3 ± 1.02	III	+	+	T4
15	Gogjalie	13.4 ± 1.37	II	+	+	-
16	Gavmishgolie	12.5 ± 0.96	II	+	+	T4
17	Ashigoland	14.1 ± 1.3	II	+	-	-
18	Gharaghshlaghe	16.4 ± 1.76	II	+	+	T4
19	Hajihassane	12.2 ± 0.88	III	+	+	-
20	Ghanzalie	11.7 ± 1.32	III	+	+	T4
21	Ghomghalad	12.4 ± 0.97	II	+	+	T4
22	Vosokandd	16.4 ± 5.02	II,I	+	+	-
23	Ghzljad	14.6 ± 0.99	II	+	+	-
24	Darmand	12.2 ± 1.2	II	+	-	-
25	Kanikozalahe	13.4 ± 1.03	III	+	+	-
26	Sardarabadd	11.6 ± 1.47	II	+	+	T4
27	Kanirashd	11.1 ± 0.98	II	+	-	-

^aValues are expressed as mean ± SD.

^bPussard and Pons’ criteria (1977).

^cBuk 11isolate, Accession no. KP940446.

^dSamples have been taken from Simineh Rud River

^eSamples have been taken from Zarineh Rud River