Table 2.

Coverage and Shannon Index calculated using MOTHUR1 for each methanogen 16S rRNA gene clone library

Clone library	OTUs observed	CHAO1 OTU estimate	% OTU coverage2	Shannon index ± 95% confidence limits	Libshuff analysis
Piglet-C	10	10	99.49	1.47 ± 0.15a	P < 0.0001
Piglet-P	11	11.5	98.95	1.53 ± 0.15b	P < 0.0001
Finisher-C	10	10	99.48	1.66 ± 0.13c	P < 0.0001
Finisher-P	15	15	99.49	2.08 ± 0.16d	P < 0.0001

¹Schloss et al

²Good’s coverage (C) according to the equation C = 1 − (n/N), where n is the number of sequences represented by a single clone and N is the total

number of clones in the library

^a,b,c,d There is significant difference between these values