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. 2017 Jan 17;7:40484. doi: 10.1038/srep40484

Figure 3. The IFNγ-triggered activations of STAT1 and −3 are attenuated by GA.

Figure 3

(A and B) Rat primary astrocytes were mock-treated or treated with the indicated concentration of GA, and then treated with 10 U/ml IFNγ for 0.5 h or 24 h. Western blot analysis was performed using the indicated antibodies. The data presented are representative of at least three independent experiments. (C) Primary astrocytes were transfected with 8 × GAS luciferase reporter plasmids, and then mock-treated or treated with 10 μg/ml GA for 1 h. The cells were mock-treated or treated with 10 U/ml IFNγ for 24 h. Cell extracts were then subjected to a luciferase activity assay. The results shown represent the mean ± SD of triplicate experiments and are representative of three individual experiments.