Western blot (A) and densitometric analysis (B) of membrane proteins from RL tumors detected with different dilutions of purified SV1 antiserum. Lanes: M, Precision Plus molecular mass marker; 1, SV1 antiserum at a 1:1,000 dilution; 2, SV1 antiserum at a 1:2,000 dilution; 3, SV1 antiserum at a 1:4,000 dilution; 4, protein omitted from Western blot; 5, SV1 antiserum omitted from Western blot; 6, secondary anti-rabbit antibody omitted from Western blot; 7, crude membrane extract from RL tumor probed with anti-actin serum.