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. 2017 Jan 17;16:9. doi: 10.1186/s12934-017-0626-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — UPLC-qTOF-HR-MS/MS analysis of the extracts obtained from the mutant strains. a UPLC-qTOF-MS/MS chromatogram selected for m/z = 527.3014 corresponding to milbemycin A3 (A3), m/z = 541.3171 corresponding to milbemycin A4 (A4) and milbemycin B2 (B2), m/z = 555.3327 corresponding to milbemycin D (D) and milbemycin B3 (B3), m/z = 569.3484 corresponding to milbemycin G (G), and m/z = 789.5158 corresponding to oligomycin A (O), respectively, of culture extracts from the S. avermitilis SAMA13 and SAMA1M7 strains. b UPLC-qTOF-MS/MS chromatogram selected for m/z = 527.3014, 541.3171, 555.3327, and 789.5158 corresponding to milbemycin A3 (A3), milbemycin A4 (A4), milbemycin D (D), and oligomycin A (O), respectively, of culture extracts from the S. avermitilis SAMA1M7ΔD strain