Table 1. Wolbachia-induced cytoplasmic incompatibility in two transinfected lines of the medfly C. capitata.
| Cross (females × males) | Embryos scored | Embryonic mortality, % |
|---|---|---|
| No tetracycline* | ||
| Uninfected × WolMed 88.6 (wCer2) | 3,000 | 100 ± 0 |
| Uninfected × WolMed S10.3 (wCer4) | 3,000 | 100 ± 0 |
| WolMed 88.6 (wCer2) × Uninfected | 3,000 | 16.73 ± 0.68 |
| WolMed S10.3 (wCer4) × Uninfected | 3,000 | 32.03 ± 0.85 |
| WolMed S10.3 (wCer4) × WolMed 88.6 (wCer2) | 3,000 | 100 ± 0 |
| WolMed 88.6 (wCer2) × WolMed S10.3 (wCer4) | 3,000 | 100 ± 0 |
| WolMed 88.6 (wCer2) × WolMed 88.6 (wCer2) | 3,000 | 64.77 ± 0.87 |
| WolMed S10.3 (wCer4) × WolMed S10.3 (wCer4) | 3,000 | 67.25 ± 0.87 |
| Uninfected × Uninfected | 3,000 | 12.17 ± 0.60 |
| Tetracycline† | ||
| WolMed S10.3 tet × WolMed S10.3 tet‡ | 1,890 | 23.44 ± 0.97 |
| WolMed S10.3 tet × WolMed S10.3 tet§ | 3,000 | 11.80 ± 0.59 |
| WolMed 88.6 tet × WolMed 88.6 tet‡ | 2,283 | 25.10 ± 0.91 |
Wolbachia-induced cytoplasmic incompatibility is expressed as percentage of unhatched eggs ± SE. Egg-laying plates were removed daily for a period of 6 days. Hatching rates were scored 72 h after egg collection.
*
Test crosses between each transinfected line and the parental naturally uninfected Benakeion strain as well as between the two transinfected lines. Crosses between 100 females (2-3 days old) and equal numbers of males (1 day old) were performed in cages.
†
Test crosses between tetracycline-treated individuals of the line WolMed S10.3. Crosses between 30 females and equal numbers of males were performed in cages, three generations (‡) and five generations (§) after the tetracycline treatment.