Table 2. Hematologic analysis in aged Sca+/AE mice.
| Differential
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype | WBC, 103/μl | Hb, g/dl | Plt, 103/μl | ANC, 103/μl | Early/mid | Late | Erythroid | Lymphoid | M/E | Retics,* % | Spleen weight,† g |
| Sca+/+ (n = 12) | 4.26 ± 0.51 | 13.9 ± 0.62 | 895 ± 71 | 1,101 ± 107 | 5.6 ± 0.4 | 60.1 ± 2.7 | 32.6 ± 2.3 | 1.8 ± 0.2 | 2.2:1 | 3.1 ± 0.2 | 0.15 ± 0.02 |
| Sca+/AE (n = 12) | 9.99 ± 1.68‡ | 13.0 ± 0.56 | 1,069 ± 119 | 4,702 ± 1,000‡ | 6.0 ± 0.7 | 74.4 ± 2.0§ | 18.5 ± 2.2§ | 1.0 ± 0.1 | 5.1:1§ | 6.5 ± 2.0 | 0.27 ± 0.04¶ |
Peripheral blood counts were obtained from consecutive, unselected pairs of littermate mice (age 9-12 months). Bone marrow differential counts and myeloid/erythroid (M/E) ratios were determined from manual 200-cell counts of May-Grünwald/Giemsa-stained bone marrow slides. Results are displayed as mean values ± SEM. Early/mid, myeloblast-myelocyte; late, metamyelocyte-neutrophil. Plt, platelets; ANC, absolute neutrophil count.
*
Sca+/+, n = 9; Sca+/AE, n = 10.
†
Sca+/+, n = 23; Sca+/AE, n = 18.
‡
P < 0.005.
§
P < 0.001.
¶
P < 0.05.