Table 3.
aResponders/Cured | bImmune to Rechallenge | cIFN-γ Mean Spots ± SD | ||
---|---|---|---|---|
# Mice/Total | Percent | |||
Control | 0/7 | 0% | 0/7 | N/A |
Radiation | 0/8 | 0% | 0/8 | N/A |
Vaccine + Rad (Schedule 2) | 4/8 | 50% | 4/4 | 147.2 ± 7.8 |
To test for abscopal effect, established Renca-MUC1tumors were treated either with vehicle (control), 8Gy radiation or vaccine followed by radiation (schedule 2, Fig. 2a). aResponders/Cured: Responders were characterized by inhibition of tumor growth or complete tumor regression. bAt an early time point after treatment, 7 days after radiation and one day after the second vaccine treatment, mice were rechallenged with Renca-MUC1 cells on the contralateral left flank. cOn day 70, the splenocytes of mice immune to rechallenge were tested in IFN-γ ELISPOT assay