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. 2017 Jan 17;16:11. doi: 10.1186/s12943-017-0582-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Mir-141 is frequently upregulated in advanced ovarian cancers. a A graph showing 18 miRNAs with the highest expression in microRNA profiling analysis which was performed using the miRCURY LNA™ miRNA array (Exiqon) on two types of ovarian cancer cell lines; anchorage-growth independent (A2780s, A2780cp, SKOV3, C13*), and anchorage-growth dependent (HOSEs). The dot line shows the miRNAs with more than 2 folds of Delta LogMedianRatios were selected for further functional analysis using transfection of their pre-miRNA expressing plasmids in ovarian cancer cell lines e.g. A2780cp and SKOV3 and performing of soft agar assay. b Real time qPCR analysis showed that miR-141 is upregulated in ovarian cancer cell lines (N = 12) as compared with the immortalized cell lines, HOSEs, (N = 3). Samples were normalized with SNORD48. c Graphical representation of qPCR analysis showing that miR-141 is elevated in ovarian cancer (N = 65) when compare to the control normal ovary tissues (N = 26) and immortalized cell lines (N = 3). d Representative pictures showing miR-141 ISH expression signal (dark blue staining) is gradually increased along the tumor stages of epithelial ovarian cancer. (Magnification × 10; scale bar, 100 μm)