Fig. 4.

Multiple sequence alignment of selected deacetylases. The alignment encompasses the entire HDAC8 protein and residues 1–375 of HDAC1 (out of 482), 1–373 of HDLP (out of 375), and 643–1056 of HDAC4 (out of 1080). HDAC8 secondary structure and residue numbering are shown above the alignment. Red vertical bar, identical residues; blue vertical bar, conserved residues; green vertical bar, residues coordinating Zn²⁺ ion; filled violet circles, residues coordinating K⁺ ions at site 1; open violet circles, residues coordinating K⁺ ions at site 2; black stars, residues contacting the inhibitor; black triangles, residues lining the internal cavity.