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. 2017 Jan 17;3:16053. doi: 10.1038/celldisc.2016.53

Figure 4.

Figure 4

The lightly assembled RecA nucleoflaments facilitate strand exchange. (a, b) CE-LIFP electropherograms of the products of strand exchange reactions in the presence of ATP. TMR-ss83mer (a) or TMR-ds83mer (b) was used as the detectable DNA probe to indicate the formation of heteroduplex TMR-ds83mer (a) or displaced ssDNA TMR-ss83mer (b). The reactions contained 3.0 μM RecA protein, 10 nM ss83mer, 10 nM ds83mer and ATP (0–1.0 mM). (c, d) CE-LIFP electropherograms of the lightly assembled nucleofilaments (c) and the strand exchange reactions (d) with RecA at low concentrations (0.1–1.0 μM). TMR-ss83mer (c) or TMR-ds83mer (d) was used as a fluorescent probe to indicate the formation of detectable lightly assembled nucleofilaments (c) or displaced TMR-ss83mer (d). ATP was kept at 1.0 mM, and 83mer DNA was kept at 10 nM. Peaks 1–3 represent the lightly assembled, moderately assembled and RecA-saturated nucleofilaments, respectively. The reactions proceeded at 37 °C for 10 min.