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. 2017 Jan 17;7:40633. doi: 10.1038/srep40633

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) In vivo tumourigenesis assay. Clones P5B3, P6D4, P2B9, P4B6 and parental OPCT-1 were injected subcutaneously into the right flanks of male athymic nude mice (6 animals per cell line). Tumour growth was monitored using calliper measurements and mice were euthanised once one of the tumours reached 1 cm in diameter. Data are presented as the mean tumour area ± SD. Statistical significance was calculated using the Student’s t-test (significance indicated as asterisks). (b) Representative tumours excised from tumour-bearing mice arranged in descending order of the clones’ in vitro vimentin positivity. (c) Immunofluorescent staining of tumour sections derived from clones P5B3, P6D4, P2B9, P4B6 and parental OPCT-1 for E-cadherin (red) and vimentin (green) expression. Representative images. (×10, ×20 and ×40 magnification, n = 3). Scale bar: 50 μM.