Figure 4. Three-dimensional reconstruction of wing epithelial cells double stained with SYBR Green I for nuclei and MitoTracker Red for mitochondria.

Vertical length of the 3D cuboid is shown in each panel. (A,B) Focal region. The apical layer (indicated as “a”) contains most mitochondria and some nuclei (arrows), whereas the basal layer (indicated as “b”) contains some nuclei (arrowheads). The distinction between these two layers is solely for convenience, but these two layers are indistinguishable in the other regions. Mitochondria form a cluster of inverted cone in a cell. (C) Adjacent region. (D,E) Basal region. Cells are tilted toward the distal direction. (F) Mitochondrial depth (the deepest mitochondrial location) from the apical surface. Shown are mean values ± standard deviation. The number of cells examined (n) and the number of pupae used (N) are indicated.