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. 2017 Jan 17;7:40635. doi: 10.1038/srep40635

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Relative abundances of methanogenic archaeal 16S rRNA genes (a) and methanotrophic bacterial pmoA genes (b) based on quantitative PCR in the paddy soil of the two treatments during the rice cultivation period (days 17, 47 and 67 after early rice transplantation). Vertical bars indicate the standard deviation of the means (n = 3). Different letters above error bars denote a significant difference between the two treatments at the same sampling time (n = 3, p < 0.05, T-test). NPK, rice cropping without dual cropping of Azolla under recommended fertilization; NPK + Azolla, rice cropping with dual cropping of Azolla under recommended fertilization.