Table 1.

PCR primer concentrations and cycling conditions

	Primer forward (50 pM/μl)	Primer reverse (50 pM/μl)	Cycling conditions
BNP	1 μl	1 μl	95°C for 3 min
			94°C for 30 s, 58°C for 30 s, 72°C for 1 min; for 40 cycles
			72°C for 7 min