. Author manuscript; available in PMC: 2017 Jan 17.

Published in final edited form as: Biochemistry. 2015 Sep 17;54(38):5949–5958. doi: 10.1021/acs.biochem.5b00678

Table 3.

Capacity and affinity of α-crystallins for T4L mutants

α-crystallin	T4L	[T4L] µM	N (± s.d.)	K_D (± s.d.) µM

human αA	D70N	3	0.20^*	14.65 (3.21)
	L99A		0.27 (0.09)	2.43 (1.42)
	L99A/130S		1.25^*	2.37 (0.31)

rat αA^ins	D70N	3	0.20^*	6.82 (1.03)
	L99A		0.33 (0.01)	0.017 (0.017)
	L99A/130S		0.32 (0.03)	0.064 (0.059)

zebrafish αA	D70N	5	0.20^*	88.92 (31.04)
	L99A		0.33^*	8.16 (1.07)
	L99A/130S		1.25^*	6.20 (0.44)

zebrafish αBa-S	D70N	3	0.20^*	1.87 (0.31)
	L99A		0.47 (0.04)	0.058 (0.057)
	L99A/130S		0.65 (0.05)	0.063 (0.060)

zebrafish αBb	D70N	3	0.20^*	4.85 (0.87)
	L99A		0.20 (0.04)	1.34 (0.17)
	L99A/130S		0.33 (0.02)	0.12 (0.06)

n was fixed during the fitting routine. See Experimental Procedures