. Author manuscript; available in PMC: 2017 Jan 17.

Published in final edited form as: Biochemistry. 2015 Sep 17;54(38):5949–5958. doi: 10.1021/acs.biochem.5b00678

Table 4.

Temperature dependence of zebrafish α-crystallin binding activity

α-crystallin	T4L	[T4L] µM	Temp (°C)	N (± s.d.)	K_D (± s.d.) µM

αA	L99A	5	28	0.20^*	50.71 (12.69)
	L99A	5	37	0.33^*	8.16 (1.07)
	L99A/130S	5	28	0.20^*	20.87 (3.82)
	L99A/130S	5	37	1.25^*	6.20 (0.44)

αBa-S	L99A	5	28	0.20 (0.04)	0.92 (0.71)
	L99A	3	37	0.64 (0.05)	0.012 (0.034)
	L99A/130S	5	28	0.28 (0.02)	0.21 (0.12)
	L99A/130S	3	37	0.65 (0.05)	0.063 (0.060)

αBb	L99A	3	37	0.20 (0.04)	1.34 (0.17)
	L99A/130S	5	23	0.20^*	28.96 (5.87)
	L99A/130S	5	28	0.20^*	4.94 (0.76)
	L99A/130S	3	37	0.34 (0.02)	0.25 (0.08)

n was fixed during the fitting routine. See Experimental Procedures