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. 2017 Jan 17;7:40674. doi: 10.1038/srep40674

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Measured BRET ratios are shown in boxplots for different donor and acceptor combinations of Hs (upper panel) and Em (lower panel) coiled-coil domains as well as the MDM2:p53 positive control. Each experiment was performed in triplicate with (+) and without (−) addition of the NanoBRET™ 618 fluorescent ligand and Z’ calculated and shown for each.