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. 2017 Jan 17;7:40674. doi: 10.1038/srep40674

Figure 8. E. muelleri phenotypes following knockdown of EmMBD2.

Figure 8

(a and b) Control and EmMBD2/3 RNAi-treated sponges photographed from above on stereomicroscope. (a) Control sponges were grown in Strekal’s media alone or soaked in dsRNA to GFP. (b) Sponges treated with dsRNA to EmMBD2/3. Leading edge of growth of basal pinacoderm (P); developing choanoderm nearest to basal pinacoderm (C), oscula (osc) and gemmule (g) are shown for reference. (c–f) Whole mount control and EmMBD2/3 RNAi-treated sponges. Magnified region as depicted in white boxes in A and B. Leading edge of basal pinacodermal (P) growth to left of images and between the red and pink lines. Developing choanoderm (C) is to right of pink line. (c) Control sponges show clearly delineated basal pinacoderm and proximal choanoderm development. (d) RNAi treated sponges show disorganized pinacoderm and proximal choanoderm. (e,f) Dapi staining of exact images shown in (c and d). Control and treated sponges show lack of connections (e.g., proper canals) forming between choanocyte chambers of the proximal choanoderm. Scale 1mm (a and b) and 200 μm (c–f).