. Author manuscript; available in PMC: 2017 Jan 17.

Published in final edited form as: J Bone Miner Res. 2015 May;30(5):855–868. doi: 10.1002/jbmr.2417

Table 1.

RANKL Transgene is Capable of Supporting Normal B-Cell and T-Cell Development

	RANKL^+/+	RANKL^−/−	RANKL^{−/−;Tg719}
Bone marrow
PreProB cells (B220⁺CD24^lowBP1⁻) (%)	3.6 ± 0.6	×	3.2 ± 0.7
ProB cells (B220⁺CD24⁺BP1⁻) (%)	3.8 ± 1.0	×	3.4 ± 0.9
PreB cells (B220⁺CD24⁺BP1⁺) (%)	9.4 ± 1.3	×	8.6 ± 3.0
Immature B cells (B220⁺CD24⁺IgM⁺IgD⁻) (%)	14.7 ± 2.9	×	12.6 ± 3.0
Transitional B cells (B220⁺CD24⁺IgM^highIgD^low) (%)	3.3 ± 0.6	×	2.7 ± 1.0
Early mature B cells (B220⁺CD24⁺IgM^highIgD^high) (%)	1.0 ± 0.8	×	1.1 ± 0.6
Late mature B cells (B220⁺CD24⁺IgM^lowIgD^high) (%)	1.7 ± 1.4	×	1.9 ± 0.9
CD3⁺ T cells (%)	1.9 ± 0.4	×	1.7 ± 0.6
Spleen
B220⁺IgM⁺ B cells (%)	26.6 ± 3.2	15.0 ± 4.8^*	28.6 ± 3.5
B220⁺IgD⁺ B cells (%)	40.6 ± 3.8	16.2 ± 5.2^*	43.3 ± 4.7
CD3⁺ T cells (%)	35.6 ± 5.8	37.4 ± 3.3	32.1 ± 3.1
CD4⁺ T cells (%)	21.9 ± 4.7	22.9 ± 3.3	19.4 ± 1.1
CD8⁺ T cells (%)	14.2 ± 1.4	15.0 ± 1.4	13.2 ± 1.9

Values are mean ± SD of 3 to 4 animals per genotype. All animals used in the experiments were 6-week-old female littermates. Flow cytometry analysis of the B and T cells in bone marrow and spleen were done after red blood cell lysis.

p<0.05 compared to wild-type as evaluated by one-way ANOVA.