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Characterization of Beta8 glycosylation. Relative quantitation of released glycans (Fc and Fab) by 2-aminobenzamide (2-AB)-labeled oligosaccharide profiling (A). Site-specific glycan analysis by reduced antibody LC-MS of heavy chain combined, de-convoluted mass spectra for Beta8 (B) and the aglycosylated Beta8-AG (N59Q) variant (C).
