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. 2016 Nov 11;9(1):104–113. doi: 10.1080/19420862.2016.1255390

Figure 4.

Figure 4.

Inhibition of TGF-β activation by 37E1B5 requires a complex glycan. Schematic overview of the different antibody glycoforms assessed for bioactivity and PK properties, illustrated for the binantennary glycan FA2G2S2 (A). TMLC-HeLa cell co-culture TGF-β signaling reporter assay. Relative luciferase units (RLU) from TMLC reporter cells show baseline luciferase reporter activity (“TMLC”). Relative luciferase units (RLU) from TMLC and HeLa αvβ8+ cell co-culture show maximal luciferase reporter activity (“TMLC + HeLa); and the anti-TGF-β mAb 1D11 (“anti-TGFb-1D11,” 7.5 µg/mL) effect on the TGF-β dependent luciferase reporter activity in the TMLC and HeLa αvβ8+ co-culture. Mouse IgG1 (“mIgG1,” 7.5 µg/mL) was used as the isotype control. (B). Maximum response of TGF-β dependent luciferase reporter activity by 37E1B5 variants in the TMLC co-culture assay with HeLa αvβ8+ cells. (C). Data shown is the mean ±SEM from 3 independent experiments.