Figure 6.
(R)-BPO-27 inhibits CFTR-dependent swelling of enteroids generated from human jejunum and CFTR Cl− current in planar enteroid cultures. A) Inhibition of forskolin-induced swelling of enteroids by (R)-BPO-27. Enteroids were incubated with (R)-BPO-27 for 10 min before addition of 5 µM forskolin. Enteroid swelling was quantified as areas of fluorescently stained enteroids as determined by confocal microscopy (means ± se; n = 8). B) Short-circuit current in planar cultures generated from the human enteroids. Cultures were treated with 10 µM forskolin, and then indicated concentrations of (R)-BPO-27 were added to both apical and basolateral solutions, followed by 25 µM CFTRinh-172 added to the apical solution. Representative of 2 sets of studies.