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Transcriptional activator properties of class 2 mutant ATF6[R376*]. FLAG epitope-tagged ATF6(373) and ATF6[R376*], a class 2 mutant, were transfected into HEK293 cells. ATF6 protein levels were detected by immunoblotting using anti-FLAG antibody. BiP/GRP78 protein level was detected by immunoblotting.
