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. 2016 Dec 27;114(2):E132–E141. doi: 10.1073/pnas.1619659114

Fig. 2.

Fig. 2.

Lipoamide-lipoamide dehydrogenase–coupled assay for the reduction of lipid hydroperoxides by XfOhr. Peroxidase activity was monitored by the consumption of NADH at 340 nm in the presence of XfOhr (0.05 μM), lipoamide dehydrogenase from X. fastidiosa (XfLpD, 0.5 μM), and lipoamide (50 μM) in sodium phosphate buffer (20 mM, pH 7.4) and DTPA (1 mM). (A) Dose-dependent reduction of LAOOH by XfOhr in the presence of Tween-20 (0.25%). (Inset) Plot of the rates of NADH oxidation, considering points up to 20-μM concentration of LAOOH. (B) Reduction of hydroperoxides (100 μM) in the presence of Triton X-100 (0.15%). PCOOH, phosphatidylcholine hydroperoxide. (C) Peroxidase activity of Ohr on PCOOH, measured after indicated time of treatment with PLA2.