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Intrinsic fluorescence of MtAhpE. (A) Intrinsic fluorescence of MtAhpE and XfOhr in their reduced (represented in black and blue lines, respectively) and oxidized (red and green lines, respectively) states. (B) Emission spectra of MtAhpE (2 μM) in the reduced (black line) or oxidized (red line) state by 15(S)-HpETE (1.8 μM) or in the presence of native (green line) or NEM alkylated (blue line) XfOhr (16 μM). RFU, relative fluorescence units.
