Skip to main content
. 2016 Dec 27;114(2):346–351. doi: 10.1073/pnas.1608576114

Fig. 3.

Fig. 3.

Age-dependent cerebellar atrophy in GM130-nKO mice. (A) Gross morphology of brains from adult control and GM130-nKO mice. (B and C) Nissl staining of sagittal sections of brain (B) and cerebellum (C) from 7-mo-old control and GM130-nKO mice. Black arrows in B indicate the position of the cerebellum. (Scale bar in B, 500 µm.) White arrows in C indicate Purkinje cells. The granule cell layer (GL) and molecular layer (ML) are indicated. (Scale bar in C, 50 µm.) (D) Purkinje cells (labeled with calbindin-D28K, green) in lobule X of the cerebellum of GM130-nKO and littermate control mice at age 2 wk (Upper) and 4 wk (Lower). Nuclei are stained with DAPI (blue). (Scale bar, 500 µm.) (E and F) Quantification of the Purkinje cell density and molecular layer thickness in lobules X and IX of GM130-nKO and littermate control mice at age 2 wk (E) and 4 wk (F). n = 3; **P < 0.01. Data are presented as the mean ± SD. (G) Immunohistochemical staining of the astrocyte marker glial fibrillary acidic protein (GFAP, red) and calbindin-D28K (green) in the cerebellum of control and GM130-nKO mice. (Scale bar, 20 µm.)