Fig. S5.

Purkinje cell apoptosis in the cerebellum of GM130 KO mice. (A) Immunohistochemistry of Purkinje cells in the cerebellum of control or GM130-nKO mice at P30 labeled for the apoptosis marker cleaved caspase 3, detected using fluorescence (Top, red, with calbindin-D28K in green) or histological detection (Bottom, brown, with hematoxylin in blue). Arrows indicate apoptotic cells. (Scale bars, Top 20 µm and Bottom 10 µm.) (B) Quantitation of Purkinje cell apoptosis in cerebellar lobules X and IX at P30. n = 3; ***P < 0.001. Data are presented as mean ± SD. (C) Calbindin-D28K immunohistochemical staining (green) of Purkinje cells in the cerebellum of 30-d-old GM130^−/− mouse and littermate control mouse. Nuclei are stained with DAPI (blue). Boxed region of lobule X is shown at Right. The background of the nKO panel is a composite, generated by addition of a sector on the Right side of the image. (Scale bar, Left 500 µm and Right 50 µm.)