Table S4.
Compound | Transitions monitored | CE, V | CV, V |
OH-pseudocobalamin | 659.8 → 136.1, 348.1 | 30 | 45 |
CN-pseudocobalamin | 673.3 → 136.1, 348.1, 660.3 | 30 | 45 |
Me-pseudocobalamin | 668.3 → 136.1, 348.1, 660.3 | 30 | 30 |
Ado-pseudocobalamin | 785.3 → 136.1, 348.1, 660.3 | 30 | 50 |
S-adenosyl methionine | 399.258 → 136.1, 250.2 | 26 | 2 |
CE, collision energy in volts (V); CV, cone voltage in volts (V). For the pseudocobalamins, parent masses are all of the [M+2H]2+ except OH-pseudocobalamin, which loses its OH- during ionization as shown previously (15). 136 m/z, α ligand (adenine); 348 m/z, α ligand with sugar and phosphate groups; 660 m/z, doubly charged loss of the β ligand. Bolded transitions were used for quantification.