Fig. S9.

Glycosylation on M2 did not affect flu replication. (A) Schematic overviews of M2 domain structure; the glycosites were highlighted with red line. The glycosite sequence on M2 was NDS. To study whether glycosylation on M2 affected flu replication, we changed N to G or S to I by reverse genetics. (B) Comparison of virus replication rates in MDCK cells. MDCK cells were infected with variants of flu at an MOI of 0.01. Culture fluids were collected at 24 h postinfection, and the virus titer was determined by plaque assay with MDCK cells. This result showed that these variants of flu had the same virus replication rate, suggesting that glycosylation did not affect the flu replication. Mean ± SEM of three independent experiments is shown.