Fig. S7.

Analysis of the effects of increasing amount of preformed seed fibrils on Aβ42 aggregation kinetics. (A) Aggregation kinetics of 2 μM Aβ42 solution in the absence (black) and presence of 1% (purple), 2% (red), 5% (green), and 10% (cyan) of preformed seed fibrils. Note the gradual increase in the half-time of the aggregation kinetics with increasing seed concentrations. (B) Simulated time evolution of the secondary nucleation rates corresponding to the reactions in A with increasing seed concentrations. (C) Fraction of the fibrils generated by secondary nucleation processes with respect to the total fibril amount at the different seed concentrations corresponding to the simulations in B. The continuous line represents calculations according to Eq. S1.