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. 2016 Dec 27;114(2):E237–E244. doi: 10.1073/pnas.1611307114

Fig. 1.

Fig. 1.

Appressorium morphogenesis is an S-phase-regulated developmental process in M. oryzae. (A) Micrographs to show effect of inhibition of DNA replication by 200 mM HU on appressorium formation in M. oryzae, added at 2 h postinoculation (hpi) and observed at 24 h. (Scale bar, 20 µm.) (B) Bar chart to show frequency of appressorium formation after exposure to HU. ****P < 0.0001 (unpaired Student’s t test; n = 3 experiments; spores = 300). (C) Diagram of LacO/LacI operator system. A construct containing 256 LacO repeats was integrated at a random locus in the genome. Fluorescence was visualized by expressing GFP–LacI–NLS, which binds to LacO repeats. In a prereplicative cell (G1) in which a single locus is present, a single punctum is observed, whereas in a postreplicative (S/G2) nucleus, two puncta appear. (Scale bar, 10 µm.) (D) Micrographs to show appressorium formation of Guy11, nim1I327E, cyc1nimE10, cyc1nimE6, and bim1F1673* mutants at 24 °C and 30 °C. (Scale bar, 20 µm.) (E) Bar chart to show frequency of appressorium formation by Guy11, nim1I327E, cyc1nimE10, cyc1nimE6, and bim1F1673* mutants at 24 and 30 °C. **P < 0.01; ***P < 0.001; ****P < 0.0001 (unpaired Student’s t test; n = 3 experiments; spores observed = 300).