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. 2016 Nov 28;28(12):2991–3004. doi: 10.1105/tpc.16.00186

Figure 4.

Figure 4.

Heterologous Expression of GMT1 in GlcA-IPC-Synthesizing Yeast.

(A) The proposed pathway for producing the Hex-GlcA-IPC using yeast. UGD2, hUGTrel7, IPUT1 as previously reported (Rennie et al., 2014), and GMT1 were expressed in sur1Δ yeast.

(B) Immunoblot showing expression of the GMT1 protein in yeast expressing UGD2, hUGTrel7, and IPUT1 (−) and yeast expressing UGD2, hUGTrel7, IPUT1, and GMT1 (+). The predicted size of GMT1 is 37 kD.

(C) and (D) MS/MS chromatograms showing the production of GlcA-IPC (black line) and Hex-GlcA-IPC (red dashed line) from yeast expressing UGD2, hUGTrel7, and IPUT1 (C) and yeast expressing UGD2, hUGTrel7, IPUT1, and GMT1 (D).