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. 2016 Dec 5;28(12):2974–2990. doi: 10.1105/tpc.16.00478

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Figure 4. — Subcellular Localization of CLD1.

(A) CaMV35S promoter-driven CLD1-GFP (left panel) and GFP only (right panel) constructs were transiently expressed in Arabidopsis protoplasts.

(B) Intact chloroplasts (IC) isolated from 4-week-old plants were disrupted and further separated into envelope/stroma (ES) and thylakoid (Thy) fractions for immunoblot analysis. TOC75 and Lhcb1 were markers for ES and Thy fractions, respectively. Proteins equivalent to 5 μg of chlorophyll were loaded in each lane.