Figure 5.

PLT2 Activates Promoters in Distinct Parts of the Root.

Activation of ProRCH1:erGFP (A), ProTLP8:erGFP (B), and ProYUC3:erGFP (C) expression in root tips after 0, 6, and 24 h of DEX induction of ProPLT2:PLT2-GR in 5-d-old plt1 plt2 seedlings. The 0 h time point reflects the lower expression in the plt1 plt2 mutant. The erGFP expression pattern is compared with that in a wild-type background. Expression of erGFP was quantified in representative depicted transformants and plotted in fold change of erGFP levels relative to the uninduced (0 h) situation. For each induction series, a transgenic root is followed in time and depicted using identical confocal settings and the same medial focal plane. Experiments were repeated four times (ProRCH1:erGFP), 12 times (ProTLP8:erGFP), and nine times (ProYUC3:erGFP). Arrowhead indicates QC. Bar = 50 μm.