Figure 6.

PLT2 Expression Pattern Influences the Boundary of Meristem Target Gene Expression Domains at the Root Tip.

Activation of ProTLP8:erGFP (A), ProYUC3:erGFP (B), and ProHAN:3xvYFP (C) expression in representative transgenic roots after 0, 6, and 24 h of DEX induction of PLT2-GR expressed under control of the constitutive 35S promoter in 5-d-old seedlings. For each induction series, a transgenic root is followed over time and depicted using identical confocal settings and the same medial focal plane. Experiments were repeated 26 times (ProTLP8:erGFP), 14 times (ProYUC3:erGFP), and eight times (ProHAN:3xvYFP). The lower panel depicts the green (GFP) channel only. Arrowhead indicates QC and arrow indicates shootward meristem boundary. Bar = 100 μm.