Figure 4.
Overexpression and Suppression of PGM48 in Arabidopsis and Effect on mRNA Levels during Plant Development and Senescence.
(A) Accumulation of transgenic StrepII-tagged PGM48 proteins in PG isolated from wild-type, OE, and RNAi rosettes as determined by immunoblotting using specific anti-StrepII serum.
(B) Accumulation level of endogenous and transgenic PGM48 and ABC1K1 proteins in PG isolated from wild-type, OE-1, and RNAi-1 rosettes as determined by immunoblotting. The Ponceau-stained blot is shown for loading control. 1x and 3x correspond to ∼4 and 12 µg of loaded isolated PG proteins.
(C) mRNA levels of PGM48, SAG12, SAG13, PES1, and CCD4, in rosettes of wild-type, OE-2, and RNAi-2 lines after 21, 28, 35, and 42 d growth under 18 h light/6 h dark, 130 µmol photons m−2 s−1 light intensity (for additional replicate, see Supplemental Figure 5F).