Fig. 1.

Immunolocalization of Fas and related factors in CyCL of the estrous rat. (A) Fas immunostaining in CyCL. (B) Pre-absorption of the antibody with the blocking peptide completely abolished the immunoreaction. (C) Immunoreactive Fas-positive cell types include large (black arrowheads) and small (white arrowheads) luteal cells, capillary endothelial cells (blue arrowheads) and blood derived granulocytes and macrophages (green arrowheads). Positive immunoreactions for FasL (D), Fas (E) and cleaved caspase-3 (F), the most of which were densely scattered in inflammatory-like and fibrotic sites (closed dashed lines in E) in the CyCL. Larger magnification of portions of central zone of D and F was available in Supplementary Fig. 1A and 1B, respectively, to identify the immunopositive cell types. Associated immunostaining for Fas (G), MPO (H) and CD68 (I) was also abundant to inflammatory-like and fibrotic sites (surrounded by the dashed line in G). Magnification of rectangles with dashed lines in H and I was available in Supplementary Fig. 2A and 2B, respectively. Scale bars, 50 µm (A, B), 25 µm (C), 100 µm (D–I).