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. 2016 May 17;10(7):1099–1117. doi: 10.1016/j.molonc.2016.05.001

Figure 7.

Figure 7

(A) Neither mifepristone (MF) (MF20, 20 μM; MF40, 40 μM) nor tunicamycin (TN; 2 μg/ml) impact the processing of poly‐ubiquitinated proteins ([Ub]n) in OV2008 cells. Concentrations of MG‐132—an inhibitor of the proteasome—0.5 μM or higher cause accumulation of [Ub]n (B) and lethality (C). (D) Reduced viability of OV2008 ovarian cancer cells exposed for 72 h to a range of cytostatic concentrations of MF and a fixed (0.25 μM) cytostatic dose of MG‐132 as determined using microcapillary cytometry. Lethal interaction between MG‐132 and MF for 72 h involves accumulation of [Ub]n (E), accumulation of p21cip1, and cleavage of PARP (FL = full length; C = cleaved) (F). GAPDH or β‐actin were used as loading controls. Results are representative of at least 2 independent experiments with a similar outcome.