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. Author manuscript; available in PMC: 2017 Sep 16.
Published in final edited form as: ACS Chem Biol. 2016 Jul 11;11(9):2428–2437. doi: 10.1021/acschembio.6b00539

Table 1.

Populations of different bound states of unphosphorylated and pY39 aSyn Y125F/Y133F in the presence of 3 mM 15% DOPS/25% DOPE/60% DOPC SUVs.

Populationsa KD Estimate (mM)b
Protein All bound
states		 Partly-helical
(incl. helix-1)		 Extended
helix		 All bound
states		 Extended
helix
aSyn Y125F/Y133F 0.377 ± 0.034 0.146 ± 0.093 0.232 ±
0.059		 4.9 10
pY39 aSyn Y125F/
Y133F 		0.319 ± 0.041 0.176 ± 0.088 0.143 ±
0.047		 6.4 18.4
a

Bound fractions are calculated as described in Methods in SI from Figures 5A and B.

b

Dissociation constants were estimated from one-point bound populations using equation 3 in the SI.