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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Neuropharmacology. 2016 Nov 19;114:20–33. doi: 10.1016/j.neuropharm.2016.11.013

Table 3.

Mutant cycle analysis of glutamate Ip EC50, Iss EC50, Iss:Ip, and ethanol IC50

Mutant (F636/F637) Ip EC50 Iss EC50 Iss:Ip Ethanol IC50
ΔΔGINT df ΔΔGINT df ΔΔGINT df ΔΔGINT df
A/A 0.44 ± 0.094**** 24 0.47 ± 0.10**** 24 0.082 ±0.079 25 −0.19 ± 0.071* 23
C/C −0.24 ± 0.054**** 27 −0.32 ± 0.067**** 27 −0.13 ± 0.064 28 −0.30 ± 0.056**** 20
I/I 0.33 ± 0.14* 24 0.42 ± 0.11** 24 −0.043 ± 0.062 24 −0.030 ± 0.061 20
L/L 0.043 ± 0.089 25 0.055 ± 0.13 25 0.46 ± 0.081**** 25 −0.12 ± 0.054* 23
W/W 0.85 ± 0.14**** 25 0.89 ± 0.11**** 25 −0.091 ± 0.048 25 −0.88 ± 0.065**** 23

Ip: peak current; Iss: steady-state current; Iss/Ip: maximal peak to steady-state current ratio; ΔΔGINT : interaction free energy; df: degrees of freedom.

Values for GINT are means ± S.E in kcal mol−1. Asterisks indicate significant differences from zero energy (one sample t- test as described in the Materials and Methods; * p < 0.05, ** p <0.01, **** p < 0.0001). Values for wild-type and single mutations used in calculations are from Ren et al. (2007, 2013).