Table 1.
GO localization and GO function of 19 GFP-Grb2 localization hits
| UniProt | GFP-Grb2 localization | GO localization | GO function | |
|---|---|---|---|---|
| AMPH | P49418 | Spots in cytoplasm | Cytoplasm, cytoskeleton, trans-Golgi | Regulation of GTPase activity |
| APBB1IP | Q7Z5R6 | Spots in cytoplasm, nucleus | Cytosplasm, cytoskeleton, plasma membrane | Signal transduction |
| BCL6 | P41182 | Small spots in nucleus | Nucleus | Regulation of GTPase activity, signaling, transcription |
| CNK2 | Q8WXI2 | Spots in cytoplasm | Cytoplasm, Golgi apparatus, membrane | Regulation of signal transduction |
| DDX17 | Q92841 | Single spot in cytoplasm | Nucleus | Regulation of transcription, RNA helicase |
| DIAPH1 | O60610 | Spots in cytoplasm | Cytoplasm, cytoskeleton, plasma membrane | Actin cytoskeleton organization |
| DOK3 | Q7L591 | Spots in cytoplasm | Cytoplasm, plasma membrane | Ras protein signal transduction |
| ENAH | Q8N8S7 | Few spots in nucleus | Cytoplasm, cytoskeleton, focal adhesion | Actin cytoskeleton organization |
| LZTS2 | Q9BRK4 | Clustered perinuclear spots | Cytoplasm, centrosome, cytoskeleton | Cell division, Wnt signaling pathway |
| MBIP | Q9NS73 | Few spots in cytoplasm | Cytoplasm, nucleus | Chromatin organization |
| PHF8 | Q9UPP1 | Few spots in cytoplasm | Nucleus | Mitotic cell cycle, chromatin organization |
| PRAM1 | Q96QH2 | Few spots in nucleus | NA | Integrin-mediated signaling pathway |
| RIMS2 | Q9UQ26 | Single spot in cytoplasm | Plasma membrane | cAMP-mediated signaling, regulation of exocytosis |
| SHKBP1 | Q8TBC3 | Clustered spots in cytoplasm | NA | Positive regulation of EGFR signaling |
| SKAP2 | O75563 | Few spots in cytoplasm | Cytoplasm, plasma membrane | Signal transduction |
| ST5 | P78524 | Few spots in cytoplasm | NA | Regulation of GTPase activity |
| TACC3 | Q9Y6A5 | Large spots in cytoplasm | Cytoplasm, microtubule, cytoskeleton | Microtubule cytoskeleton organization |
| TUFT1 | Q9NNX1 | Spots in cytoplasm, nucleus | Cytoplasm, extracellular region | Intracellular signal transduction |
| Usp33 | Q8TEY7 | Few spots in cytoplasm | Cytoplasm, cytoskeleton, Golgi | Protein deubiquitination |
NA – not annotated