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Mutation of the PY motif of Mdm34 does not affect the localization of Mdm12 and Mmm1. MDM34-MCHERRY and MDM34[3PA]-MCHERRY strains bearing chromosomal genes encoding MDM12-GFP and MMM1-GFP were grown to the exponential growth phase in glycerol-containing medium (YPG). Cells were washed twice and processed for fluorescence microscopy. Cell shape was assessed with DIC.
