Table 2. Features of tagged members of the pir, fam-a and fam-b multigene families.
| Name tagged protein | Fluorescent tag | Mutant name | RMgmDB ID1 | BLOOD | LIVER | |||
|---|---|---|---|---|---|---|---|---|
| expression and localisation | % of clones fluorescent | fluorescent before passage (%) | fluorescent after passage (%) | protein localisation | ||||
| Single gene-tagging mutants (multigene families) | ||||||||
| Fam-a1 | mCherry | 1477cl3 | 690 | Yes; RBC s | 33% (n = 3) | 80–90% | 50–70% | Yes; PV |
| Fam-a1 | GFP | 1941 | 1283 | Yes; RBC s | N.A. | 25–30% | 30% | No |
| Fam-a2 | mCherry | 1448cl2 | 693 | Yes; RBC c pa | 60% (n = 5) | 99% | 85–100% | Yes; PV |
| Fam-b1 | mCherry | 1599cl4 | 699 | Yes; RBC c pa | 66% (n = 3) | 5–15% | 5% | Yes; PV |
| Fam-b2 | mCherry | 1731cl4 | 700 | Yes; RBC c pa | 100% (n = 5) | 65% | 50–70% | Yes; PV |
| Fam-b2 | GFP | 1942 | 1282 | Yes; RBC c pa | N.A. | 50–75% | N.D. | N.D |
| PIR1 | mCherry | 1531cl3 | 695 | Yes; RBC c pa | 100% (n = 5) | 15% | 60–70% | Yes; PV |
| PIR1 | mCherry | 1944cl1 | 1281 | Yes; RBC c pa | 66% (n = 3) | 10–20% | N.D. | N.D. |
| PIR2 | GFP | 603cl3 | 696 | Yes; RBC c pa | 75% (n = 4) | 5–10% | 5% | No |
| PIR3 | mCherry | 1918cl4 | 697 | Yes; RBC c pa | 25% (n = 4) | 1%-5% | N.D. | No |
| PIR4 | mCherry | 2450 | 1233 | Yes; RBC c pa | N.A. | 0.1–2% | N.D. | No |
| PIR5 | mCherry | 2448cl1 | 1234 | Yes; RBC c pa | 100% (n = 3) | 25–50% | N.D. | N.D. |
| PIR6 | mCherry | 1892 | 698 | Yes; RBC c pa | N.A. | <0.1% | N.D. | N.D. |
| PIR7 | mCherry | 2211 | 1235 | Yes; RBC c pa | N.A. | <0.1% | N.D. | N.D. |
| PIR8 | mCherry | 2312, 2313 | 1236 | Yes; RBC c pa | N.A. | 50–60% | 30–60% | yes, parasite cyt |
| Double gene-tagging mutants (multigene families) | ||||||||
| Fam-a2 Fam-a1 Fam-a2/a1 |
mCherry GFP mCherry&GFP |
2010 (2011) | 1244 | Yes; RBC c pa Yes; RBC s |
N.A. | 70–80% 40–65% 40–55% |
70–80% 45–50% 30–40% |
Yes; PV (>90%) No |
| Fam-a2 Fam-a1 Fam-a2/a1 |
GFP mCherry GFP&mCherry |
2504cl3 | 1245 | Yes; RBC c pa Yes; RBC s |
100% (n = 3) | 80–90% 80–90% 80–85% |
75–80% 60–65% 70–80% |
Yes; PV (70–75%) Yes, PV (30–40%) 30–40% |
| Fam-b1 Fam-b2 Fam-b1/b2 |
mCherry GFP mCherry&GFP |
2421(-2424) | 1246 | Yes; RBC c pa Yes; RBC c pa |
N.A. | 40–50% 40–45% 35–45% |
30–50% 50–80% 20–40% |
Yes; PV No |
| PIR1 PIR3 PIR1/PIR3 |
mCherry GFP mCherry&GFP |
2020 (2021) | 1247 | Yes; RBC c pa Yes; RBC c pa |
N.A. | 35–45% 25–35% 20–30% |
40–60% 1–20% 1–5% |
Yes; PV No |
| Single gene-tagging mutants (single copy genes) | ||||||||
| IBIS1 | GFP mcherry |
2009 1940cl1 |
1237 | Yes; RBC c pu | N.A. 100% (n = 1) |
N.D >90% |
N.D. N.D. |
Yes; PV (>90%) |
| SMAC | mCherry | 1565cl1 | 1238 | Yes; RBC c pa | 100% (n = 4) | >90% | N.D. | Yes; PV (>90%) |