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. Author manuscript; available in PMC: 2017 Jan 17.
Published in final edited form as: Methods Mol Biol. 2016;1470:85–94. doi: 10.1007/978-1-4939-6337-9_7

Table 2.

Suggested electroporation conditions for several leukemia cell lines

Cell line Electroporation condition % viability via cell counting % viability via MTS assay
MOLT4 250 V, 2.0 ms 86 79
JURKAT 200 V, 2.5 ms 81 62
GDM-1 250 V, 4.0 ms 95 63
Kasumi-2 200 V, 4.0 ms 81 53
LOUCY 250 V, 4.0 ms 83 87
MOLT-16 250 V, 2.0 ms N/A 75
UT-7 350 V, 1.0 ms N/A 77
SUPT-13 200 V, 4.5 ms N/A 81
HBPALL 250 V, 3.5 ms N/A 85
SKW-3 200 V, 4.5 ms N/A 75
PEER 200 V, 4.5 ms N/A 90
CCRF 250 V, 3.0 ms N/A 89
RPMI-8402 250 V, 3.0 ms N/A 60
PF-382 250 V, 3.0 ms N/A 70
AML-1 250 V, 3.0 ms N/A 53
ML-1 250 V, 3.0 ms N/A 72
HL-60 250 V, 3.0 ms N/A 63
TF-1 250 V, 3.0 ms N/A 62
HT-93 300 V,3.0 ms N/A 80
Primary myeloid cells 275 V,5.0 ms
Primary lymphoid cells 250 V, 4.0 ms

Note: The electroporation conditions might slightly vary from above suggested conditions because of variation in cell clones. Therefore it is recommended to optimize own conditions around the above-given parameters