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. 2017 Jan 17;15(1):e2000094. doi: 10.1371/journal.pbio.2000094

Fig 6. The HZA enhancer mediated the transcriptional activity of HIZR-1 in animals.

Fig 6

(A) Diagrams of the basal pes-10 promoter with and without three HZA enhancer elements (not to scale) [15]. (B,C) Representative images of anterior intestine of hizr-1(am285gf) transgenic animals containing pes-10p::gfp-nls (basal pes-10) or 3XHZApes-10p::gfp-nls (basal pes-10+3X HZA); intestines are outlined, arrowheads indicate representative GFP-positive nuclei, and scale bars are approximately 25 μm. (D) hizr-1(+), hizr-1(am286lf), and hizr-1(am285gf) transgenic animals expressing 3XHZApes-10p::gfp-nls were cultured with 0 or 200 μM supplemental zinc and assayed for GFP expression. n, number of animals assayed, *, p < 0.05 compared to hizr-1(+) animals cultured with 0 μM supplemental zinc (Chi-squared test).