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. 2017 Jan 17;12(1):e0170158. doi: 10.1371/journal.pone.0170158

Fig 5. γ-secretase inhibition causes increased ER trafficking of EdU-labeled pseudogenome.

Fig 5

DMSO or XXI treated cells were infected with HPV16 PsV harboring EdU-labeled pseudogenome for 12 h (A-D) or 20 h (E-H). ER was stained with BiP (green), EdU-labeled pseudogenome was visualized by Click-it reaction (red) and the nuclei were stained with DAPI (grey). (A-D and I) DMSO treated HaCaTs showed significantly less colocalization compared to XXI treated HaCaTs at 12hpi (one-tailed t-test p = 0.008). (E-H and I) At 20hpi, significantly higher levels of colocalization were detected in XXI treated cells compared to DMSO treated cells (one-tailed t-test p = 0.02). (I) M1 coefficient, percentage of red signal (EdU-labeled pseudogenome) overlapping with green signal (BiP) was calculated with the help of JaCOP plug in with nine confocal scans for each condition. (A-H). Error bars show the standard deviation in measurements from three independent replicates.